Efektivitas Antifungi Ekstrak Daun Teh Hijau (Camellia sinensis) dengan Metode UAE terhadap Malassezia furfur secara In Vitro

Mahera A Rachmania; Yuni Setyaningsih; Adi Sukrisno; Fajriati Zulfa

doi:10.28932/jmh.v8i2.14494

Authors

Mahera A Rachmania UPN "Veteran" Jakarta
Yuni Setyaningsih Departemen Parasitologi Fakultas Kedokteran, Universitas Pembangunan Nasional “Veteran” Jakarta
Adi Sukrisno 3Departemen Obstetri dan Ginekologi Fakultas Kedokteran, Universitas Pembangunan Nasional “Veteran” Jakarta
Fajriati Zulfa Departemen Parasitologi Fakultas Kedokteran, Universitas Pembangunan Nasional “Veteran” Jakarta

DOI:

https://doi.org/10.28932/jmh.v8i2.14494

Keywords:

antifungal, green tea leaf extract, Malassezia furfur, pityriasis versicolor, ultrasonic assisted extraction

Abstract

Fungal infections in Indonesia affect 2.89% of the population and have the potential to develop resistance to azole-class antifungals, as seen in Pityriasis versicolor caused by Malassezia furfur. Green tea leaf (Camellia sinensis) extract offers a safer antifungal alternative containing tannins, flavonoids, alkaloids, and triterpenoids. This experimental study evaluated C. sinensis extract effectiveness against M. furfur in vitro using a post-test only control group design. Extracts were prepared via Ultrasonic Assisted Extraction (UAE) method at 10%, 15%, 20%, and 25%, with sterile aquadest and 2% ketokonazol as controls. Antifungal activity was assessed using the well diffusion method on Sabouraud Dextrose Agar (SDA) by measuring the inhibition zone after 24 and 48 hours of incubation. Inhibition was generally moderate at 24 hours but weakened at 48 hours, except at 20% and 25% concentrations. Bivariate analysis of 24-hour data using the Kruskal-Wallis’s test showed a significant difference in the inhibition of M. furfur among green tea leaf extract concentrations (p<0.05), with higher concentrations associated with greater inhibition. Based on post-Hoc analysis, it was found that the 20% concentration was the most effective concentration for inhibiting M.furfur.

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