THE INHIBITION EFFECT OF MORINGA (Moringa oleifera L.) LEAVES AND RED GINGER RHIZOME (Zingiber officinale Rosc.) PT. "P" ETHANOL EXTRACT ON ENHANCEMENT SERUM CREATININE LEVELS OF MALE WISTAR RATS DYSLIPIDEMIA MODEL

Juliana Wijaya; Penny S Martioso; Sugiarto Puradisastra

doi:10.28932/jmh.v5i1.4528

Authors

Juliana Wijaya Universitas Kristen Maranatha
Penny S Martioso Universitas Kristen Maranatha
Sugiarto Puradisastra Universitas Kristen Maranatha

DOI:

https://doi.org/10.28932/jmh.v5i1.4528

Keywords:

Moringa oleifera L. leaves, Zingiber officinale Rosc. Rhizome, creatinine

Abstract

Elevation of serum creatinine levels is a biomarker of decreased kidney function. Dyslipidemia can decrease kidney function. Long-term consuming statins can increase creatinine levels. Moringa leaves (Moringa oleifera L.) and red ginger rhizomes (Zingiber officinale Rosc.) have antioxidant effects. Aim of study is to determine the inhibitory effect on increasing serum creatinine levels of concoction Moringa Leaves and Red Ginger Rhizome ethanol extract (CML-RGRE). True laboratory experimental study with completely randomized design was conducted on 30 male Wistar rat dyslipidemia models, induced by high-fat feeding (HFF)-Propylthiouracil (PTU) 0.01% for 21 days. Subjects were divided into six groups (n=5), Group I, II, III were treated CML-RGRE 135, 202.5, 270 mg/kg BW, IV dyslipidemia control, V Rosuvastatin 1.8 mg/kg BW, and VI fenofibrate 5.4 mg/kg BW, all still given HFF-PTU for 21 days. Serum creatinine levels (mg/dL) were measured 3 times, after acclimatization, created dyslipidemia models, and treatment using spectrophotometer, colorimetric kinetic methods. Data were analysed with paired t-test, ANOVA test, and Fischer's LSD, α = 0.05. All doses of CML-RGRE have effectively inhibited the increase serum creatinine levels (p < 0.01). Conclusion, Moringa Leaves and Red Ginger Rhizome ethanol extract effectively inhibited the increased serum creatinine levels of animal dyslipidemia models.

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References

Wibawanto DF, Paris S, Thadeus MS. Hubungan gagal ginjal kronik tingkat 4 dan 5 dengan kejadian dislipidemia pada pasien gagal ginjal kronik di rumah sakit umum pusat fatmawati periode 2016. J Agric Chem Soc Japan. 2016;70:217.

Dan HD. Hubungan dislipidemia dan kejadian penyakit jantung koroner. J Kedokteran dan Kesehatan Indonesia. 2014;6(1):1–7.

Kemenkes RI. Kementerian Kesehatan RI Badan Penelitian dan Pengembangan. Hasil Utama Riset Kesehatan Dasar. Jakarta: Kementrian Kesehatan Republik Indonesia; 2018.

Connell AW, Sowers JR. The CardioRenal Metabolic Syndrome [Internet]. NCBI. 2014. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4170524/

Liu DW, Wan J, Liu ZS, Pei Wang P, Yang G, Cheng XZS. Association between dyslipidemia and chronic kidney disease: a cross-sectional study in the middle-aged and elderly Chinese population [Internet]. [cited 2021 Jan 25]. Available from: https://pubmed.ncbi.nlm.nih.gov/23557545/

Virginia DM. Dislipidemia sebagai faktor risiko penurunan nilai estimasi laju filtrasi glomerulus (eLFG) pada diabetes mellitus tipe II (Dyslipidemia as a risk factor of declining estimated glomerular filtration rate (eGFR) value on diabetes mellitus type II). Jurnal Ilmu Kefarmasian Indonesia. 2015;13(1):17-22.

Rubba P, Marotta G, Gentile M. Efficacy, and safety of rosuvastatin in the management of dyslipidemia. Vasc Health Risk Management. 2009; 5:343-52.

PERKENI. Arsana PM, Rosandi R, Manaf A, Budhiarta A, Permana H, Sucipta KW, et al. (Eds.). Pengelolaan P. Panduan pengelolaan dislipidemia. Jakarta: PB PERKENI; 2015.

PERKI. Erwinanto E, Santoso A., Putranto J.N.E., Tedjasukmana P., Suryawan R., Rifqi S, et al. (Eds.). Pedoman tatalaksana dislipidemia PERKI 2013. Indones J Cardiol. 2015;34(4):245–70.

Han E, Kim G, Lee JY, Lee YH, Kim BS, Lee BW, et al. Comparison between atorvastatin and rosuvastatin in renal function decline among patients with diabetes. Endocrinol Metab. 2017;32(2):274–80.

BPOM. Fibrat. Badan Pengawas Obat dan Makanan. 2015 [cited 2021 Oct 4]. Available from: http://pionas.pom.go.id/ioni/bab-2-sistem-kardiovaskuler-0/210-hipolipidemik/2103-fibrat

Ni Nyoman Yuliani, Jefrin Sambara MAM. Uji aktivitas antioksidan fraksi etilasetat ekstrak etanol rimpang jahe merah (Zingiber officinale var. Rubrum) dengan metode DPPH (1,1-Diphenyl-2- Picrylhydrazyl). Inf Kesehat. 2016;14.

Pakpahan TL. Manfaat jahe (Zingiber officinale Roscoe) terhadap kadar asam urat. J Agromedicine [Internet].2015;2(4):530–5. Available from: http://juke.kedokteran.unila.ac.id/index.php/agro/ article/view/1250/pdf

Pratama Putra I, Dharmayudha A, Sudimartini L. Identifikasi senyawa kimia ekstrak etanol daun kelor (Moringa oleifera L) di Bali. Indones Med Veterinus. 2017;5(5):464–73.

Saryono S. Efek daun alpukat (Persea Americana M.) dan daun kelor (Moringa oleifera L.) terhadap peningkatan kadar hdl model tikus putih hiperlipidemia. J Keperawatan Soedirman.2017;12(1):47.

Kamaliani BR, Setiasih NLE, Winaya IBO. Histopathological kidney overview of experimental diabetes mellitus wistar rats given ethanol extract of moringa leaf. Bul Vet Udayana. 2019;(21):71.

Al AM, Abukhalil MH, Alghonmeen RD, Mahmoud AM. Ginger alleviates hyperglycemia-induced oxidative stress, inflammation and apoptosis and protects rats against diabetic nephropathy Biomedicine & Pharmacotherapy Ginger alleviates hyperglycemia-induced oxidative stress inflammation and apoptosis and pr. Biomed Pharmacother [Internet]. 2018;106:381–9. Available from: https://doi.org/10.1016/j.biopha.2018.06.148

Harsa IMS. Efek pemberian diet tinggi lemak terhadap profil lemak darah tikus putih (Rattus norvegicus). Ilmu Kedokteran. 2014;3(1):21–8.

Parwata MOA. Bahan ajar antioksidan. Kimia Terapan Program Pascasarjana Universitas Udayana. 2016;(4):1–54.

Alverina C, Andari D, Prihanti GS. Pengaruh pemberian ekstrak daun kelor (Moringa oleifera lam.) terhadap sel kardiomiosit pada tikus putih (Rattus novergicus strain wistar) dengan diet aterogenik. Saintika Med. 2016;12(1):30.

Depkes RI. Pedoman pengujian dan pengembangan fitofarmaka, penapisan farmakologi, pengujian fitokimia dan pengujian klinik. Jakarta: Depkes RI. 1993.

Martha Veronika, Ekawati Purwijantiningsih, Sinung Pranata. Efektivitas ekstrak daun kelor (Moringa oleifera) sebagai bio-sanitizer tangan dan daun selada (Lactuca sativa). Yogyakarta: F. Teknobiologi-Program Studi Biologi, Univ. Atma Jaya. 2017;6-22.

Elina Januarini. Optimasi formulasi minuman fungsional terintegrasi daun kelor, daun pandan wangi, dan jahe merah (Moringa oleifera Lamk, Pandanus maryllifolius Roxb, dan Zingiber officinale Rosc.). Malang: Jurusan Teknologi Hasil Pertanian – Fakultas Teknologi Pertanian, Universitas Brawijaya. 2017;6-10.

Hasan IC, Subroto H, Puspasari G. Bitter leaves ethanol extract (Vernonia amygdalina) decreases total cholesterol serum level of hypercholesterolemic male wistar rats. J Med Heal.2018;2(2):764–71.