Pengaruh Ekstrak Kunyit dan Kulit Manggis Terhadap Ekspresi Gen PPARα Pada Retina Model Tikus yang Dinduksi DTL
DOI:
https://doi.org/10.28932/jmh.v5i2.6684Kata Kunci:
retina, HFD, metabolic dysfunctionAbstrak
Diet tinggi lemak (DTL) dalam waktu lama telah terbukti menginduksi disfungsi metabolik dan menyebabkan inflamasi berbagai organ, termasuk retina. Sebagai regulator metabolisme lipid, PPARa memegang peran penting dalam metabolisme lipid dan berperan sebagai salah satu target penurunan deposisi lipid di retina. Kunyit dan kulit manggis merupakan obat tradisional Indonesia dengan efek Kesehatan yang luas, termasuk berkhasiat sebagai anti-inflamasi dan hipolipidemik. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol kunyit dan kulit manggis terhadap ekspresi gen PPARa pada retina tikus model yang diinduksi DTL. Dua puluh ekor tikus Wistar Jantan dibagi menjadi 5 kelompok, yaitu kelompok kontrol negative, kontrol positif, kunyit, manggis, dan fibrat. Pada akhir penelitian, retina diekstraksi untuk dilakukan PCR realtime untuk pemeriksaan ekspresi gen PPARa. Hasil penelitian menunjukkan adanya perbedaan bermakna ekspresi gen PPARa pada retina antara kelompok kontrol negatif, positif, dan kunyit, namun tidak ditemukan perbedaan bermakna pada kelompok lainnya. Peningkatan ekspresi gen PPARa oleh kurkumin menunjukkan potensinya untuk mencegah kerusakan retina akibat DTL dalam waktu lama. Penelitian ini menyimpulkan bahwa ekstrak kunyit meningkatkan ekspresi gen PPARa pada retina tikus model induksi DTL.Unduhan
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Hak Cipta (c) 2023 Yenny Noor, Diana K Jasaputra, Julia Gunadi, Ronny Lesmana, Riska A Safira
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